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Cytometric measurement of the DNA cell cycle in the presence of
chlorophyll autofluorescence in marine eukaryotic phytoplankton
by the blue-light excited dye YoYo-1
by
Frank J. Jochem and Doris Meyerdierks
Marine
Ecology Progress Series 185: 301-307; 1999
ABSTRACT
The novel blue-light
excited fluorescent DNA dye YOYO-1® was tested on cultures of 8 eukaryotic
phytoplankton species from the classes of Prymnesiophyceae, Bacillariophyceae,
Coscinodiscophyceae, Prasinophyceae and Dinophyceae, and the coccoid cyanobacterium
Synechococcus sp. In all tested species, YOYO staining of formalin
fixed cells allowed to differentiate cell cycle phases of G1, S, and G2+M
in the presence of chlorophyll autofluorescence in flow cytometric analyses.
Diel cell cycle patterns of Emiliania huxleyi and Skeletonema
costatum grown under light/dark cycle and continuous light could be
established. Under light/dark cycle, DNA replication occurred during the
night and cell division in the early morning in both species. S. costatum
spent, however, a longer fraction of their cell cycle in S and G2+M phases
as compared to E. huxleyi. Changes in cellular chlorophyll content
and cell size could be observed in relation to cell cycle phasing. Cell
division phasing was nearly lost under continuous light. Growth rates calculated
from DNA cell cycle analyses agreed well with those established from cell
counts.
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